Review



sh sy5y human neuroblastoma cell lines  (ATCC)


Bioz Verified Symbol ATCC is a verified supplier
Bioz Manufacturer Symbol ATCC manufactures this product  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
    • 99
      Buy from Supplier

    Structured Review

    ATCC sh sy5y human neuroblastoma cell lines
    Abcam ab64186 recognizes NRN1 protein in mouse neuroblastoma cells. (A) Representative Western blot of human embryonic kidney (HEK) 293T, Neuro‐2a (N2a) mouse neuroblastoma, <t>and</t> <t>SH‐SY5Y</t> human neuroblastoma cell lysates (50 µg protein), probed with NRN1 polyclonal antibody Abcam ab64186 and GAPDH monoclonal antibody. (B) N2a cells were transfected with NRN1 or Scramble (non‐targeting) siRNA smart pools and harvested after 96 h. Western blot analyses with NRN1 polyclonal antibody Abcam ab64186 revealed reduced intensity of the ∼34 kDa band in NRN1‐depleted cells, compared to Scramble control. 10 µg of protein was loaded per lane, and GAPDH was probed as a loading control. GAPDH, glyceraldehyde‐3‐phosphate dehydrogenase; NRN1, Neuritin‐1; siRNA, small interfering RNA;
    Sh Sy5y Human Neuroblastoma Cell Lines, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 9741 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/sh sy5y human neuroblastoma cell lines/product/ATCC
    Average 99 stars, based on 9741 article reviews
    sh sy5y human neuroblastoma cell lines - by Bioz Stars, 2026-02
    99/100 stars

    Images

    1) Product Images from "NRN1 as a therapeutic target for Alzheimer's disease"

    Article Title: NRN1 as a therapeutic target for Alzheimer's disease

    Journal: Alzheimer's & Dementia

    doi: 10.1002/alz.71149

    Abcam ab64186 recognizes NRN1 protein in mouse neuroblastoma cells. (A) Representative Western blot of human embryonic kidney (HEK) 293T, Neuro‐2a (N2a) mouse neuroblastoma, and SH‐SY5Y human neuroblastoma cell lysates (50 µg protein), probed with NRN1 polyclonal antibody Abcam ab64186 and GAPDH monoclonal antibody. (B) N2a cells were transfected with NRN1 or Scramble (non‐targeting) siRNA smart pools and harvested after 96 h. Western blot analyses with NRN1 polyclonal antibody Abcam ab64186 revealed reduced intensity of the ∼34 kDa band in NRN1‐depleted cells, compared to Scramble control. 10 µg of protein was loaded per lane, and GAPDH was probed as a loading control. GAPDH, glyceraldehyde‐3‐phosphate dehydrogenase; NRN1, Neuritin‐1; siRNA, small interfering RNA;
    Figure Legend Snippet: Abcam ab64186 recognizes NRN1 protein in mouse neuroblastoma cells. (A) Representative Western blot of human embryonic kidney (HEK) 293T, Neuro‐2a (N2a) mouse neuroblastoma, and SH‐SY5Y human neuroblastoma cell lysates (50 µg protein), probed with NRN1 polyclonal antibody Abcam ab64186 and GAPDH monoclonal antibody. (B) N2a cells were transfected with NRN1 or Scramble (non‐targeting) siRNA smart pools and harvested after 96 h. Western blot analyses with NRN1 polyclonal antibody Abcam ab64186 revealed reduced intensity of the ∼34 kDa band in NRN1‐depleted cells, compared to Scramble control. 10 µg of protein was loaded per lane, and GAPDH was probed as a loading control. GAPDH, glyceraldehyde‐3‐phosphate dehydrogenase; NRN1, Neuritin‐1; siRNA, small interfering RNA;

    Techniques Used: Western Blot, Transfection, Control, Small Interfering RNA



    Similar Products

    sh sy5y human neuroblastoma cell lines  (ATCC)
    99
    ATCC sh sy5y human neuroblastoma cell lines
    Abcam ab64186 recognizes NRN1 protein in mouse neuroblastoma cells. (A) Representative Western blot of human embryonic kidney (HEK) 293T, Neuro‐2a (N2a) mouse neuroblastoma, <t>and</t> <t>SH‐SY5Y</t> human neuroblastoma cell lysates (50 µg protein), probed with NRN1 polyclonal antibody Abcam ab64186 and GAPDH monoclonal antibody. (B) N2a cells were transfected with NRN1 or Scramble (non‐targeting) siRNA smart pools and harvested after 96 h. Western blot analyses with NRN1 polyclonal antibody Abcam ab64186 revealed reduced intensity of the ∼34 kDa band in NRN1‐depleted cells, compared to Scramble control. 10 µg of protein was loaded per lane, and GAPDH was probed as a loading control. GAPDH, glyceraldehyde‐3‐phosphate dehydrogenase; NRN1, Neuritin‐1; siRNA, small interfering RNA;
    Sh Sy5y Human Neuroblastoma Cell Lines, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/sh sy5y human neuroblastoma cell lines/product/ATCC
    Average 99 stars, based on 1 article reviews
    sh sy5y human neuroblastoma cell lines - by Bioz Stars, 2026-02
    99/100 stars
    		  Buy from Supplier
    human neuroblastoma cell line sh sy5y  (ATCC)
    99
    ATCC human neuroblastoma cell line sh sy5y
    Abcam ab64186 recognizes NRN1 protein in mouse neuroblastoma cells. (A) Representative Western blot of human embryonic kidney (HEK) 293T, Neuro‐2a (N2a) mouse neuroblastoma, <t>and</t> <t>SH‐SY5Y</t> human neuroblastoma cell lysates (50 µg protein), probed with NRN1 polyclonal antibody Abcam ab64186 and GAPDH monoclonal antibody. (B) N2a cells were transfected with NRN1 or Scramble (non‐targeting) siRNA smart pools and harvested after 96 h. Western blot analyses with NRN1 polyclonal antibody Abcam ab64186 revealed reduced intensity of the ∼34 kDa band in NRN1‐depleted cells, compared to Scramble control. 10 µg of protein was loaded per lane, and GAPDH was probed as a loading control. GAPDH, glyceraldehyde‐3‐phosphate dehydrogenase; NRN1, Neuritin‐1; siRNA, small interfering RNA;
    Human Neuroblastoma Cell Line Sh Sy5y, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/human neuroblastoma cell line sh sy5y/product/ATCC
    Average 99 stars, based on 1 article reviews
    human neuroblastoma cell line sh sy5y - by Bioz Stars, 2026-02
    99/100 stars
    		  Buy from Supplier
    sh sy5y human neuroblastoma cell line sh sy5y cells  (ATCC)
    99
    ATCC sh sy5y human neuroblastoma cell line sh sy5y cells
    Abcam ab64186 recognizes NRN1 protein in mouse neuroblastoma cells. (A) Representative Western blot of human embryonic kidney (HEK) 293T, Neuro‐2a (N2a) mouse neuroblastoma, <t>and</t> <t>SH‐SY5Y</t> human neuroblastoma cell lysates (50 µg protein), probed with NRN1 polyclonal antibody Abcam ab64186 and GAPDH monoclonal antibody. (B) N2a cells were transfected with NRN1 or Scramble (non‐targeting) siRNA smart pools and harvested after 96 h. Western blot analyses with NRN1 polyclonal antibody Abcam ab64186 revealed reduced intensity of the ∼34 kDa band in NRN1‐depleted cells, compared to Scramble control. 10 µg of protein was loaded per lane, and GAPDH was probed as a loading control. GAPDH, glyceraldehyde‐3‐phosphate dehydrogenase; NRN1, Neuritin‐1; siRNA, small interfering RNA;
    Sh Sy5y Human Neuroblastoma Cell Line Sh Sy5y Cells, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/sh sy5y human neuroblastoma cell line sh sy5y cells/product/ATCC
    Average 99 stars, based on 1 article reviews
    sh sy5y human neuroblastoma cell line sh sy5y cells - by Bioz Stars, 2026-02
    99/100 stars
    		  Buy from Supplier
    human sh sy5y neuroblastoma cell line  (ATCC)
    99
    ATCC human sh sy5y neuroblastoma cell line
    Abcam ab64186 recognizes NRN1 protein in mouse neuroblastoma cells. (A) Representative Western blot of human embryonic kidney (HEK) 293T, Neuro‐2a (N2a) mouse neuroblastoma, <t>and</t> <t>SH‐SY5Y</t> human neuroblastoma cell lysates (50 µg protein), probed with NRN1 polyclonal antibody Abcam ab64186 and GAPDH monoclonal antibody. (B) N2a cells were transfected with NRN1 or Scramble (non‐targeting) siRNA smart pools and harvested after 96 h. Western blot analyses with NRN1 polyclonal antibody Abcam ab64186 revealed reduced intensity of the ∼34 kDa band in NRN1‐depleted cells, compared to Scramble control. 10 µg of protein was loaded per lane, and GAPDH was probed as a loading control. GAPDH, glyceraldehyde‐3‐phosphate dehydrogenase; NRN1, Neuritin‐1; siRNA, small interfering RNA;
    Human Sh Sy5y Neuroblastoma Cell Line, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/human sh sy5y neuroblastoma cell line/product/ATCC
    Average 99 stars, based on 1 article reviews
    human sh sy5y neuroblastoma cell line - by Bioz Stars, 2026-02
    99/100 stars
    		  Buy from Supplier
    human neuroblastoma cell line shsy5y  (ATCC)
    99
    ATCC human neuroblastoma cell line shsy5y
    Abcam ab64186 recognizes NRN1 protein in mouse neuroblastoma cells. (A) Representative Western blot of human embryonic kidney (HEK) 293T, Neuro‐2a (N2a) mouse neuroblastoma, <t>and</t> <t>SH‐SY5Y</t> human neuroblastoma cell lysates (50 µg protein), probed with NRN1 polyclonal antibody Abcam ab64186 and GAPDH monoclonal antibody. (B) N2a cells were transfected with NRN1 or Scramble (non‐targeting) siRNA smart pools and harvested after 96 h. Western blot analyses with NRN1 polyclonal antibody Abcam ab64186 revealed reduced intensity of the ∼34 kDa band in NRN1‐depleted cells, compared to Scramble control. 10 µg of protein was loaded per lane, and GAPDH was probed as a loading control. GAPDH, glyceraldehyde‐3‐phosphate dehydrogenase; NRN1, Neuritin‐1; siRNA, small interfering RNA;
    Human Neuroblastoma Cell Line Shsy5y, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/human neuroblastoma cell line shsy5y/product/ATCC
    Average 99 stars, based on 1 article reviews
    human neuroblastoma cell line shsy5y - by Bioz Stars, 2026-02
    99/100 stars
    		  Buy from Supplier
    sh sy5y human neuroblastoma cell line  (ATCC)
    99
    ATCC sh sy5y human neuroblastoma cell line
    Gene expression changes following CTHRC1 manipulation <t>in</t> <t>SH-SY5Y</t> human neuroblastoma cells. Relative gene expression changes following CTHRC1 (A) siRNA knockdown or (B) overexpression compared to control. Data are presented as mean ± SEM. Statistical significance was determined by t -test (* p < 0.05, ** p < 0.01, *** p < 0.001; ns, not significant). Green bars represent control, whereas orange bars represent CTHRC1 knockdown or overexpression. (C) Western blotting analysis following CTHRC1 overexpression in SH-SY5Y cells. β -actin was used as a control. EV: empty vector.
    Sh Sy5y Human Neuroblastoma Cell Line, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/sh sy5y human neuroblastoma cell line/product/ATCC
    Average 99 stars, based on 1 article reviews
    sh sy5y human neuroblastoma cell line - by Bioz Stars, 2026-02
    99/100 stars
    		  Buy from Supplier
    sk n be 2 human neuroblastoma cell line  (ATCC)
    96
    ATCC sk n be 2 human neuroblastoma cell line
    Gene expression changes following CTHRC1 manipulation <t>in</t> <t>SH-SY5Y</t> human neuroblastoma cells. Relative gene expression changes following CTHRC1 (A) siRNA knockdown or (B) overexpression compared to control. Data are presented as mean ± SEM. Statistical significance was determined by t -test (* p < 0.05, ** p < 0.01, *** p < 0.001; ns, not significant). Green bars represent control, whereas orange bars represent CTHRC1 knockdown or overexpression. (C) Western blotting analysis following CTHRC1 overexpression in SH-SY5Y cells. β -actin was used as a control. EV: empty vector.
    Sk N Be 2 Human Neuroblastoma Cell Line, supplied by ATCC, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/sk n be 2 human neuroblastoma cell line/product/ATCC
    Average 96 stars, based on 1 article reviews
    sk n be 2 human neuroblastoma cell line - by Bioz Stars, 2026-02
    96/100 stars
    		  Buy from Supplier

    Image Search Results


    Abcam ab64186 recognizes NRN1 protein in mouse neuroblastoma cells. (A) Representative Western blot of human embryonic kidney (HEK) 293T, Neuro‐2a (N2a) mouse neuroblastoma, and SH‐SY5Y human neuroblastoma cell lysates (50 µg protein), probed with NRN1 polyclonal antibody Abcam ab64186 and GAPDH monoclonal antibody. (B) N2a cells were transfected with NRN1 or Scramble (non‐targeting) siRNA smart pools and harvested after 96 h. Western blot analyses with NRN1 polyclonal antibody Abcam ab64186 revealed reduced intensity of the ∼34 kDa band in NRN1‐depleted cells, compared to Scramble control. 10 µg of protein was loaded per lane, and GAPDH was probed as a loading control. GAPDH, glyceraldehyde‐3‐phosphate dehydrogenase; NRN1, Neuritin‐1; siRNA, small interfering RNA;

    Journal: Alzheimer's & Dementia

    Article Title: NRN1 as a therapeutic target for Alzheimer's disease

    doi: 10.1002/alz.71149

    Figure Lengend Snippet: Abcam ab64186 recognizes NRN1 protein in mouse neuroblastoma cells. (A) Representative Western blot of human embryonic kidney (HEK) 293T, Neuro‐2a (N2a) mouse neuroblastoma, and SH‐SY5Y human neuroblastoma cell lysates (50 µg protein), probed with NRN1 polyclonal antibody Abcam ab64186 and GAPDH monoclonal antibody. (B) N2a cells were transfected with NRN1 or Scramble (non‐targeting) siRNA smart pools and harvested after 96 h. Western blot analyses with NRN1 polyclonal antibody Abcam ab64186 revealed reduced intensity of the ∼34 kDa band in NRN1‐depleted cells, compared to Scramble control. 10 µg of protein was loaded per lane, and GAPDH was probed as a loading control. GAPDH, glyceraldehyde‐3‐phosphate dehydrogenase; NRN1, Neuritin‐1; siRNA, small interfering RNA;

    Article Snippet: SH‐SY5Y human neuroblastoma cell lines (Catalog No.: CRL‐2266, ATCC) were maintained in a 1:1 mixture of DMEM/F12 (Catalog No.: 11330057, Thermo Fisher Scientific) and Eagle's Minimum Essential Medium (Catalog No.: 50‐238‐2632, Fisher Scientific) with 10% FBS and 1% penicillin‐streptomycin.

    Techniques: Western Blot, Transfection, Control, Small Interfering RNA

    Gene expression changes following CTHRC1 manipulation in SH-SY5Y human neuroblastoma cells. Relative gene expression changes following CTHRC1 (A) siRNA knockdown or (B) overexpression compared to control. Data are presented as mean ± SEM. Statistical significance was determined by t -test (* p < 0.05, ** p < 0.01, *** p < 0.001; ns, not significant). Green bars represent control, whereas orange bars represent CTHRC1 knockdown or overexpression. (C) Western blotting analysis following CTHRC1 overexpression in SH-SY5Y cells. β -actin was used as a control. EV: empty vector.

    Journal: Frontiers in Aging Neuroscience

    Article Title: Identification of CTHRC1 as a novel candidate for neurodevelopmental disorders

    doi: 10.3389/fnagi.2026.1737003

    Figure Lengend Snippet: Gene expression changes following CTHRC1 manipulation in SH-SY5Y human neuroblastoma cells. Relative gene expression changes following CTHRC1 (A) siRNA knockdown or (B) overexpression compared to control. Data are presented as mean ± SEM. Statistical significance was determined by t -test (* p < 0.05, ** p < 0.01, *** p < 0.001; ns, not significant). Green bars represent control, whereas orange bars represent CTHRC1 knockdown or overexpression. (C) Western blotting analysis following CTHRC1 overexpression in SH-SY5Y cells. β -actin was used as a control. EV: empty vector.

    Article Snippet: The SH-SY5Y human neuroblastoma cell line was obtained from ATCC (CRL-2266).

    Techniques: Gene Expression, Knockdown, Over Expression, Control, Western Blot, Plasmid Preparation